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ERGIC3 Monoclonal antibody

ERGIC3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B9F11

Cat no : 68398-1-Ig

Synonyms

C20orf47, CGI 54, dJ477O4.2, ERGIC and golgi 3, ERGIC3, Erv46, NY BR 84, PRO0989, SDBCAG84

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ERGIC3 Monoclonal antibody (68398-1-Ig)

    WB analysis using 68398-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68398-1-Ig (ERGIC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inNCI-H1299 cells, Calu-3 cells, U2OS cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68398-1-Ig targets ERGIC3 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ERGIC3 fusion protein Ag9190
Full Name ERGIC and golgi 3
Calculated Molecular Weight 383 aa, 43 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC009765
Gene Symbol ERGIC3
Gene ID (NCBI) 51614
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERGIC3 (Endoplasmic reticulum-Golgi intermediate compartment protein 3 ) is located in the cis face of the Golgi apparatus and vesicular tubular structures between the transitional endoplasmic reticulum (ER) and cis-Golgi. ERGIC3 significantly affects cell growth and causes ER stress-induced cell death, and is involved in the invasion and metastasis in hepatocellular carcinomas (HCC). (PMID: 26177443, PMID: 31142615)

Protocols

Product Specific Protocols
WB protocol for ERGIC3 antibody 68398-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols