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  • Western Blot (WB) analysis of various lysates using ACTRT3/ARPM1 Monoclonal antibody (68372-1-Ig)

    WB analysis using 68372-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68372-1-Ig (ARPM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ACTRT3/ARPM1 Monoclonal antibody (68372-1-Ig)

    WB analysis using 68372-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68372-1-Ig (ARPM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using ACTRT3/ARPM1 Monoclonal antibody (68372-1-Ig)

    IHC staining of human colon using 68372-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 68372-1-Ig (ARPM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ACTRT3/ARPM1 Monoclonal antibody

ACTRT3/ARPM1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68372-1-Ig
Clone No.3B2B10

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, rabbit

Applications

WB, IHC, ELISA

Synonyms

actin related protein M1, ARPM1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inNCCIT cells, Rabbit testis, Rat testis, Human testis
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68372-1-Ig targets ACTRT3/ARPM1 in WB, IHC, ELISA applications and shows reactivity with Human, rat, rabbit samples.

Tested Reactivity Human, rat, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ACTRT3/ARPM1 fusion protein Ag33162 Predict reactive species
Full Name actin related protein M1
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC007289
Gene Symbol ACTRT3/ARPM1
Gene ID (NCBI) 84517
RRIDAB_3085092
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BYD9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ACTRT3/ARPM1 antibody 68372-1-IgDownload protocol
IHC protocol for ACTRT3/ARPM1 antibody 68372-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols