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CCDC50 Monoclonal antibody

CCDC50 Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2F12G7

Cat no : 68369-1-Ig

Synonyms

YMER, Protein Ymer, DFNA44, Coiled-coil domain-containing protein 50, C3orf6

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CCDC50 Monoclonal antibody (68369-1-Ig)

    WB analysis using 68369-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68369-1-Ig (CCDC50 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Flow cytometry (FC) experiment of A549 cells using CCDC50 Monoclonal antibody (68369-1-Ig)

    FC experiment of A549 using 68369-1-Ig

    1x10^6 A549 cells were intracellularly stained with 0.4 ug CCDC50 Monoclonal antibody (68369-1-Ig, Clone:2F12G7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

68369-1-Ig targets CCDC50 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CCDC50 fusion protein Ag15889
Full Name coiled-coil domain containing 50
Calculated Molecular Weight 482 aa, 56 kDa
Observed Molecular Weight 36-38 kDa
GenBank Accession NumberBC065004
Gene Symbol CCDC50
Gene ID (NCBI) 152137
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCDC50 encodes Ymer, an effector of epidermal growth factor (EGF)-mediated cell signaling that is ubiquitously expressed in different organs and has been suggested to inhibit down-regulation of the EGF receptor. CCDC50 act as a multifunctional regulator in NF-κB and Fas signaling pathways. In chronic lymphocytic leukaemia (CLL) and mantle cell lymphoma (MCL), CCDC50 is required for the survival of MCL and CLL cancer cells. Recently, CCDC50 was shown to promote hepatocellular carcinoma through the Ras/Foxo4 signaling pathway. (PMID: 34453126, PMID: 32612200, PMID: 17503326)

Protocols

Product Specific Protocols
WB protocol for CCDC50 antibody 68369-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO Rep

RNF144B negatively regulates antiviral immunity by targeting MDA5 for autophagic degradation

Authors - Guoxiu Li
View Article