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MCTS1 Monoclonal antibody

MCTS1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F10C7

Cat no : 68360-1-Ig

Synonyms

Multiple copies T-cell malignancies, MCT-1, MCT1, Malignant T-cell-amplified sequence 1, 1F10C7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MCTS1 Monoclonal antibody (68360-1-Ig)

    WB analysis using 68360-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68360-1-Ig (MCTS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MCTS1 Monoclonal antibody (68360-1-Ig)

    IF Staining of U2OS using 68360-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using MCTS1 antibody (68360-1-Ig, Clone: 1F10C7 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MCTS1 Monoclonal antibody (68360-1-Ig)

    IF Staining of HeLa using 68360-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MCTS1 antibody (68360-1-Ig, Clone: 1F10C7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inU2OS cells, HeLa cells, Raji cells, Jurkat cells, K-562 cells, MOLT-4 cells
Positive IF/ICC detected inU2OS cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68360-1-Ig targets MCTS1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MCTS1 fusion protein Ag7057
Full Name malignant T cell amplified sequence 1
Calculated Molecular Weight 21 kDa
Observed Molecular Weight19 kDa
GenBank Accession NumberBC001013
Gene Symbol MCTS1
Gene ID (NCBI) 28985
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCTS1 is an anti-oncogene that plays a role in cell cycle regulation. It can reduce cell doubling time and anchor-dependent growth. The G1 transition time and the duration of the G1 / S transition are shortened. It can also promote the release of deacylated tRNA and mRNA from recycled 40S subunits following ABCE1-mediated dissociation of post-termination ribosomal complexes into subunits (PMID: 10440924).

Protocols

Product Specific Protocols
WB protocol for MCTS1 antibody 68360-1-IgDownload protocol
IF protocol for MCTS1 antibody 68360-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols