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TXNDC9 Monoclonal antibody

TXNDC9 Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2A10G3

Cat no : 68335-1-Ig

Synonyms

Thioredoxin domain-containing protein 9, Protein 1-4, Protein 1 4, PHLP3, ATP-binding protein associated with cell differentiation

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TXNDC9 Monoclonal antibody (68335-1-Ig)

    WB analysis using 68335-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68335-1-Ig (TXNDC9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of HEK-293 cells using TXNDC9 Monoclonal antibody (68335-1-Ig)

    WB analysis of HEK-293 using 68335-1-Ig

    WB result of TXNDC9 antibody (68335-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TXNDC9 transfected HEK-293 cells.

  • Flow cytometry (FC) experiment of HEK-293 cells using TXNDC9 Monoclonal antibody (68335-1-Ig)

    FC experiment of HEK-293 using 68335-1-Ig

    1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug TXNDC9 Monoclonal antibody (68335-1-Ig, Clone:2A10G3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inU2OS cells, HEK-293 cells, LNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68335-1-Ig targets TXNDC9 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TXNDC9 fusion protein Ag33259
Full Name thioredoxin domain containing 9
Calculated Molecular Weight 226 aa, 27 kDa
Observed Molecular Weight27 kDa
GenBank Accession NumberBC005968
Gene Symbol TXNDC9
Gene ID (NCBI) 10190
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TXNDC9, also known as APACD, has a negative effect on protein folding by significantly reducing the activity of chaperone protein TCP1 complex ATPase activity. Including actin or tubulin.

Protocols

Product Specific Protocols
WB protocol for TXNDC9 antibody 68335-1-IgDownload protocol
FC protocol for TXNDC9 antibody 68335-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols