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ZNHIT3 Monoclonal antibody

ZNHIT3 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1C7A3

Cat no : 68331-1-Ig

Synonyms

HNF 4a coactivator, TR interacting protein 3, TRIP 3, TRIP3, zinc finger, HIT type 3, ZNHIT3

Validation Data Gallery

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  • Western Blot (WB) analysis of hTERT-RPE1 cells using ZNHIT3 Monoclonal antibody (68331-1-Ig)

    WB analysis of hTERT-RPE1 using 68331-1-Ig

    hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 68331-1-Ig (ZNHIT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ZNHIT3 Monoclonal antibody (68331-1-Ig)

    WB analysis using 68331-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68331-1-Ig (ZNHIT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of T-47D cells using ZNHIT3 Monoclonal antibody (68331-1-Ig)

    WB analysis of T-47D using 68331-1-Ig

    T-47D cells were subjected to SDS PAGE followed by western blot with 68331-1-Ig (ZNHIT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ZNHIT3 Monoclonal antibody (68331-1-Ig)

    IF Staining of HeLa using 68331-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ZNHIT3 antibody (68331-1-Ig, Clone: 1C7A3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inhTERT-RPE1 cells, Jurkat cells, T-47D cells, MOLT-4 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68331-1-Ig targets ZNHIT3 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ZNHIT3 fusion protein Ag29872
Full Name zinc finger, HIT type 3
Calculated Molecular Weight 155 aa, 18 kDa
Observed Molecular Weight18 kDa
GenBank Accession NumberBC017931
Gene Symbol ZNHIT3
Gene ID (NCBI) 9326
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNHIT3, also named TRIP3, belongs to the zinc finger HIT (Zf-HIT) domain-containing proteins family. ZNHIT3 encodes a nuclear zinc finger protein previously implicated in transcriptional regulation and small nucleolar ribonucleoprotein particle assembly and thus possibly to pre-ribosomal RNA processing (PMID: 28335020). ZNHIT3 contains at least two domains: a ZN-HIT domain that consists of a double zinc-finger, probably involved in protein−protein interaction, and a PAC-HIT domain that folds into a clamp able to trap an α-helix, here again of about 20 residues, located in the sequence of NUFIP1 (PMID: 35315277). ZNHIT3 has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for ZNHIT3 antibody 68331-1-IgDownload protocol
IF protocol for ZNHIT3 antibody 68331-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols