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EPS8L2 Monoclonal antibody

EPS8L2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2E3B1

Cat no : 68330-1-Ig

Synonyms

EPS8 like 2, EPS8 like protein 2, EPS8 related protein 2, EPS8L2, EPS8R2, FLJ16738, FLJ21935, FLJ22171

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using EPS8L2 Monoclonal antibody (68330-1-Ig)

    WB analysis of A431 using 68330-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 68330-1-Ig (EPS8L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using EPS8L2 Monoclonal antibody (68330-1-Ig)

    WB analysis of HepG2 using 68330-1-Ig

    WB result of EPS8L2 antibody (68330-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPS8L2 transfected HepG2 cells.

  • Western Blot (WB) analysis of rat skin tissue using EPS8L2 Monoclonal antibody (68330-1-Ig)

    WB analysis of rat skin using 68330-1-Ig

    rat skin tissue were subjected to SDS PAGE followed by western blot with 68330-1-Ig (EPS8L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EPS8L2 Monoclonal antibody (68330-1-Ig)

    IF Staining of HeLa using 68330-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EPS8L2 antibody (68330-1-Ig, Clone: 2E3B1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inrat skin tissue, A431 cells, HepG2 cells, A549 cells，LNCaP cells，HeLa cells，HepG2 cells, mouse skin tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68330-1-Ig targets EPS8L2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EPS8L2 fusion protein Ag16808
Full Name EPS8-like 2
Calculated Molecular Weight 715 aa, 81 kDa
Observed Molecular Weight81-85 kDa
GenBank Accession NumberBC002474
Gene Symbol EPS8L2
Gene ID (NCBI) 64787
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EPS8L2 (epidermal growth factor receptor pathway substrate 8 L2), a member of the Eps8-like protein family, is a newly identified hair bundle protein that is localized at the tips of stereocilia of both cochlear and vestibular hair cells.

Protocols

Product Specific Protocols
WB protocol for EPS8L2 antibody 68330-1-IgDownload protocol
IF protocol for EPS8L2 antibody 68330-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols