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LRRC8C Monoclonal antibody

LRRC8C Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4A6A4

Cat no : 68323-1-Ig

Synonyms

AD158, DKFZp586J1119, FAD158, LRRC8C

Validation Data Gallery

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  • Western Blot (WB) analysis of U2OS cells using LRRC8C Monoclonal antibody (68323-1-Ig)

    WB analysis of U2OS using 68323-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 68323-1-Ig (LRRC8C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using LRRC8C Monoclonal antibody (68323-1-Ig)

    WB analysis using 68323-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68323-1-Ig (LRRC8C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LRRC8C Monoclonal antibody (68323-1-Ig)

    IF Staining of HepG2 using 68323-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LRRC8C antibody (68323-1-Ig, Clone: 4A6A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

Tested Applications

Positive WB detected inHeLa cells, U2OS cells, HEK-239 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68323-1-Ig targets LRRC8C in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen LRRC8C fusion protein Ag17001
Full Name leucine rich repeat containing 8 family, member C
Calculated Molecular Weight 803 aa, 92 kDa
Observed Molecular Weight105 kDa
GenBank Accession NumberBC113973
Gene Symbol LRRC8C
Gene ID (NCBI) 84230
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LRRC8C (Leucine-rich repeat-containing protein 8C), also known as AD158, is a non-essential component of the volume-regulated anion channel (VRAC, also known as VSOAC channel), which is required to maintain a constant cell volume in response to extracellular or intracellular permeability changes. The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine. The VRAC channel also mediates transport of immunoreactive cyclic dinucleotide GMP-AMP (2'-3'-cGAMP), an immune messenger produced in response to DNA virus in the cytosol (PMID:24790029; 26824658; 28193731).

Protocols

Product Specific Protocols
WB protocol for LRRC8C antibody 68323-1-IgDownload protocol
IF protocol for LRRC8C antibody 68323-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols