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NAB2 Monoclonal antibody

NAB2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F1E2

Cat no : 68322-1-Ig

Synonyms

EGR 1 binding protein 2, MADER, NAB2, NGFI A binding protein 2, Protein MADER

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NAB2 Monoclonal antibody (68322-1-Ig)

    WB analysis using 68322-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68322-1-Ig (NAB2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NAB2 Monoclonal antibody (68322-1-Ig)

    WB analysis using 68322-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68322-1-Ig (NAB2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHEK-293 cells, U2OS cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells, A2780 cells, A375 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68322-1-Ig targets NAB2 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NAB2 fusion protein Ag6346
Full Name NGFI-A binding protein 2 (EGR1 binding protein 2)
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC065931
Gene Symbol NAB2
Gene ID (NCBI) 4665
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NGFI-A-Binding protein 2 (NAB2) is a member of the poly(A)-binding protein (PABP) family in S. cerevisiae and emerged as a key component in the tightly intertwined system of poly(A) tail length control, mRNP formation, and export of mature mRNPs.

Protocols

Product Specific Protocols
WB protocol for NAB2 antibody 68322-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols