SNX18 Monoclonal antibody
SNX18 Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
2E7D6
Cat no : 68305-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HCT 116 cells, NIH/3T3 cells, A549 cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells |
Positive IF/ICC detected in | A431 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
68305-1-Ig targets SNX18 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.
Tested Reactivity | Human, Mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | SNX18 fusion protein Ag17388 |
Full Name | sorting nexin 18 |
Calculated Molecular Weight | 628 aa, 69 kDa |
Observed Molecular Weight | 67-70 kDa |
GenBank Accession Number | BC060791 |
Gene Symbol | SNX18 |
Gene ID (NCBI) | 112574 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX18, together with SNX9 and SNX33, constitutes a separate SNX9 subfamily which is required for mitosis through endocytosis processes (PMID: 22718350, 20427313). SNX18 acts as a positive regulator of autophagy, and regulates ATG9A trafficking from recycling endosomes (PMID: 24113029, 29437695).
Protocols
Product Specific Protocols | |
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WB protocol for SNX18 antibody 68305-1-Ig | Download protocol |
IF protocol for SNX18 antibody 68305-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |