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ATP5F1 Monoclonal antibody

ATP5F1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A10G9

Cat no : 68304-1-Ig

Synonyms

ATP5F1, ATPase subunit b, PIG47

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP5F1 Monoclonal antibody (68304-1-Ig)

    WB analysis using 68304-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68304-1-Ig (ATP5F1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATP5F1 Monoclonal antibody (68304-1-Ig)

    IF Staining of HeLa using 68304-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ATP5F1 antibody (68304-1-Ig, Clone: 1A10G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68304-1-Ig targets ATP5F1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATP5F1 fusion protein Ag8571
Full Name ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1
Calculated Molecular Weight 256 aa, 29 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC005366
Gene Symbol ATP5F1
Gene ID (NCBI) 515
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP5F1(ATP synthase subunit b) belongs to the eukaryotic ATPase B chain family. The ATP5F1 gene encodes subunit B of the mitochondrial ATP synthase Fo unit, which contains 214-amino acid with a 42-amino acid import signal(PMID:1831354). Mitochondrial membrane ATP synthase(F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain.

Protocols

Product Specific Protocols
WB protocol for ATP5F1 antibody 68304-1-IgDownload protocol
IF protocol for ATP5F1 antibody 68304-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols