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PAK4 Monoclonal antibody

PAK4 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2G6A3

Cat no : 68298-1-Ig

Synonyms

KIAA1142, p21 activated kinase 4, PAK 4, PAK4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PAK4 Monoclonal antibody (68298-1-Ig)

    WB analysis using 68298-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68298-1-Ig (PAK4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PAK4 Monoclonal antibody (68298-1-Ig)

    IF Staining of HeLa using 68298-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PAK4 antibody (68298-1-Ig, Clone: 2G6A3 ) at dilution of 1:3000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, MCF-7 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:1500-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68298-1-Ig targets PAK4 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PAK4 fusion protein Ag6364
Full Name p21 protein (Cdc42/Rac)-activated kinase 4
Calculated Molecular Weight 64 kDa
Observed Molecular Weight64 kDa
GenBank Accession NumberBC025282
Gene Symbol PAK4
Gene ID (NCBI) 10298
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAK4(p21-activated kinase 4) is also named as KIAA1142. It belongs to the protein kinase superfamily.PAK4 regulates cell morphology, cytoskeletal organization, and cell proliferation and migration. It can also function as an antiapoptotic protein (PMID:14517283). It has 4 isoforms produced by alternative splicing. And it can be autophosphorylated on serine residues when activated by CDC42/p21 (PMID:11278822).

Protocols

Product Specific Protocols
WB protocol for PAK4 antibody 68298-1-IgDownload protocol
IF protocol for PAK4 antibody 68298-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols