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MATR3 Monoclonal antibody

MATR3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3B12C7

Cat no : 68280-1-Ig

Synonyms

DKFZp686K0542, DKFZp686K23100, KIAA0723, MATR3, matrin 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MATR3 Monoclonal antibody (68280-1-Ig)

    WB analysis using 68280-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68280-1-Ig (MATR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of various lysates using MATR3 Monoclonal antibody (68280-1-Ig)

    WB analysis using 68280-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68280-1-Ig (MATR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inLNCaP cells, rat brain tissue, A431 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68280-1-Ig targets MATR3 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MATR3 fusion protein Ag31502
Full Name matrin 3
Calculated Molecular Weight 847 aa, 95 kDa
Observed Molecular Weight120 kDa
GenBank Accession NumberBC015031
Gene Symbol MATR3
Gene ID (NCBI) 9782
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MATR3, known as KIAA0723, is an 847 amino acid protein, which may play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. MATR3 was originally identified as a nuclear matrix protein containing both DNA and RNA binding domains (PMID: 2033075) with a wide variety of functions ranging from DNA damage response to transcriptional and post-transcriptional regulation of cellular and viral RNAs(PMID: 21771346). the calculated molecular weight of MATR3 is 94 kDa, but we detect a 125 kDa protein as a result of PMID: 28977530.

Protocols

Product Specific Protocols
WB protocol for MATR3 antibody 68280-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols