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MAP4 Monoclonal antibody

MAP4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1C1A6

Cat no : 68271-1-Ig

Synonyms

Microtubule-associated protein 4, MAP-4, MAP 4, 1C1A6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAP4 Monoclonal antibody (68271-1-Ig)

    WB analysis using 68271-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68271-1-Ig (MAP4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MAP4 Monoclonal antibody (68271-1-Ig)

    IHC staining of human lung cancer using 68271-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68271-1-Ig (MAP4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MAP4 Monoclonal antibody (68271-1-Ig)

    IHC staining of human lung cancer using 68271-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68271-1-Ig (MAP4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using MAP4 Monoclonal antibody (68271-1-Ig)

    IF Staining of A431 using 68271-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using MAP4 antibody (68271-1-Ig, Clone: 1C1A6 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inNCCIT cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, MOLT-4 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68271-1-Ig targets MAP4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MAP4 fusion protein Ag1741
Full Name microtubule-associated protein 4
Calculated Molecular Weight 121 kDa
Observed Molecular Weight210-240 kDa
GenBank Accession NumberBC012794
Gene Symbol MAP4
Gene ID (NCBI) 4134
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAP4 is a ubiquitously expressed microtubule-associated protein involved in the organization and stabilization of microtubules during various cellular activities. Recently it has been reported that expression of MAP4 was upregulated in esophageal squamous cell carcinoma (ESCC). MAP4 has been considered as an independent prognostic factor for ESCC. The predicted molecular weight of MAP4 is about 120 kDa, while higher molecular weight around 200-250 kDa is usually observed in WB test, which may be the result of glycosylation. (26876215, 8647865)

Protocols

Product Specific Protocols
WB protocol for MAP4 antibody 68271-1-IgDownload protocol
IHC protocol for MAP4 antibody 68271-1-IgDownload protocol
IF protocol for MAP4 antibody 68271-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols