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CARD11 Monoclonal antibody

CARD11 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3F5H6

Cat no : 68269-1-Ig

Synonyms

BIMP3, CARD11, Carma 1, CARMA1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CARD11 Monoclonal antibody (68269-1-Ig)

    WB analysis using 68269-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68269-1-Ig (CARD11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using CARD11 Monoclonal antibody (68269-1-Ig)

    WB analysis of Raji using 68269-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 68269-1-Ig (CARD11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using CARD11 Monoclonal antibody (68269-1-Ig)

    IF Staining of L02 using 68269-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using CARD11 antibody (68269-1-Ig, Clone: 3F5H6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inMOLT-4 cells, Raji cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68269-1-Ig targets CARD11 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CARD11 fusion protein Ag16468
Full Name caspase recruitment domain family, member 11
Calculated Molecular Weight 1154 aa, 133 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC111719
Gene Symbol CARD11
Gene ID (NCBI) 84433
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CARD11 also named as ,BIMP3 or CARMA1, is a 1154 amino acid protein, which contains one PDZ domain, one guanylate kinase-like domain, and one CARD domain. CARD11 localizes in the cytoplasm and co-localized with DPP4 in membrane raft. CARD11 is detected in adult peripheral blood leukocytes, thymus, spleen and liver. CARD11 also is found in CARD11 promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. CARD11 is involved in the co-stimulatory signal essential for T-cell receptor mediated T-cell activation.

Protocols

Product Specific Protocols
WB protocol for CARD11 antibody 68269-1-IgDownload protocol
IF protocol for CARD11 antibody 68269-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols