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TMPRSS2 Monoclonal antibody

TMPRSS2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H7E9

Cat no : 68264-1-Ig

Synonyms

FLJ41954, PP9284, PRSS10, Serine protease 10, TMPRSS2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMPRSS2 Monoclonal antibody (68264-1-Ig)

    WB analysis using 68264-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68264-1-Ig (TMPRSS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TMPRSS2 Monoclonal antibody (68264-1-Ig)

    WB analysis using 68264-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68264-1-Ig (TMPRSS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of pig kidney tissue using TMPRSS2 Monoclonal antibody (68264-1-Ig)

    WB analysis of pig kidney using 68264-1-Ig

    pig kidney tissue were subjected to SDS PAGE followed by western blot with 68264-1-Ig (TMPRSS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inPC-3 cells, pig kidney tissue, LNCaP cells, Caco-2 cells, COLO 320 cells, HT-29 cells, human testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68264-1-Ig targets TMPRSS2 in WB, ELISA applications and shows reactivity with Human, Pig samples.

Tested Reactivity Human, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TMPRSS2 fusion protein Ag5824
Full Name transmembrane protease, serine 2
Calculated Molecular Weight 54 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC051839
Gene Symbol TMPRSS2
Gene ID (NCBI) 7113
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMPRSS2, also named as PRSS10, is a type II transmembrane serine protease which is highly expressed by the epithelium of the human prostate gland. TMPRSS2 may contribute to prostate tumour metastasis via the activation of PAR-2. TMPRSS2 is a Serine protease that proteolytically cleaves and activates the viral spike glycoproteins which facilitate virus-cell membrane fusions. TMPRSS2 is a host cell factor that is critical for the spread of several clinically relevant viruses, including influenza A viruses and coronaviruses (PMID: 23468491, 30626688). SARS-CoV-2 uses the SARS-CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming. The initial spike protein priming by TMPRSS2 is essential for the entry and viral spread of SARS-CoV-2 through interaction with the ACE2 receptor (PMID: 32142651, 30626688 ). Camostat mesylate, an inhibitor of TMPRSS2, can block SARS-CoV-2 infection of lung cells (PMID: 32142651). The MW of TMPRSS2 is about 65-70 kDa. It can be cleaved into some chains with MW 54 kDa, 31 kDa and 26 kDa (PMID: 25734995, 20382709, 26018085).

Protocols

Product Specific Protocols
WB protocol for TMPRSS2 antibody 68264-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols