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ZER1 Monoclonal antibody

ZER1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1H9G7

Cat no : 68258-1-Ig

Synonyms

C9orf60, Hzyg, Protein zer 1 homolog, RP11 545E17.4, zer 1 homolog (C. elegans), ZER1, ZYG, Zyg 11 homolog B like protein, ZYG11BL

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ZER1 Monoclonal antibody (68258-1-Ig)

    WB analysis of Jurkat using 68258-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68258-1-Ig (ZER1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using ZER1 Monoclonal antibody (68258-1-Ig)

    WB analysis of LNCaP using 68258-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68258-1-Ig (ZER1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZER1 Monoclonal antibody (68258-1-Ig)

    IF Staining of HepG2 using 68258-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ZER1 antibody (68258-1-Ig, Clone: 1H9G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inLNCaP cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68258-1-Ig targets ZER1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ZER1 fusion protein Ag10040
Full Name zer-1 homolog (C. elegans)
Calculated Molecular Weight 766 aa, 88 kDa
Observed Molecular Weight 80-88 kDa
GenBank Accession NumberBC052563
Gene Symbol ZER1
Gene ID (NCBI) 10444
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZER1 antibody 68258-1-IgDownload protocol
IF protocol for ZER1 antibody 68258-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols