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NDUFS1 Monoclonal antibody

NDUFS1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3H5E8

Cat no : 68253-1-Ig

Synonyms

EC:7.1.1.2, Complex I-75kD, Complex I 75kD, CI-75kD, CI 75Kd

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFS1 Monoclonal antibody (68253-1-Ig)

    WB analysis using 68253-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68253-1-Ig (NDUFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

  • Western Blot (WB) analysis of HeLa cells using NDUFS1 Monoclonal antibody (68253-1-Ig)

    WB analysis of HeLa using 68253-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68253-1-Ig (NDUFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using NDUFS1 Monoclonal antibody (68253-1-Ig)

    IHC staining of mouse heart using 68253-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68253-1-Ig (NDUFS1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using NDUFS1 Monoclonal antibody (68253-1-Ig)

    IHC staining of mouse heart using 68253-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68253-1-Ig (NDUFS1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NDUFS1 Monoclonal antibody (68253-1-Ig)

    IF Staining of HeLa using 68253-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NDUFS1 antibody (68253-1-Ig, Clone: 3H5E8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NDUFS1 Monoclonal antibody (68253-1-Ig)

    IF Staining of HepG2 using 68253-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NDUFS1 antibody (68253-1-Ig, Clone: 3H5E8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inpig brain tissue, HeLa cells, rabbit brain tissue, rat brain tissue, mouse brain tissue, pig heart tissue, rat heart tissue, mouse heart tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

68253-1-Ig targets NDUFS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Cited Reactivityrat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NDUFS1 fusion protein Ag3135
Full Name NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase)
Calculated Molecular Weight 727 aa, 79 kDa
Observed Molecular Weight68-81 kDa
GenBank Accession NumberBC030833
Gene Symbol NDUFS1
Gene ID (NCBI) 4719
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The multisubunit NADH:ubiquinone oxidoreductase (75 kDa subunit, mitochondrial)(NDUFS1) is the first enzyme complex in the electron transport chain of mitochondria. It is also named as Complex I-75kD. By use of chaotropic agents, complex I can be fragmented into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. NDUFS1 is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. NDUFS1 has some isoforms with the molecular mass of 68-81 kDa.

Protocols

Product Specific Protocols
WB protocol for NDUFS1 antibody 68253-1-IgDownload protocol
IHC protocol for NDUFS1 antibody 68253-1-IgDownload protocol
IF protocol for NDUFS1 antibody 68253-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Arch Oral Biol

Correlation of disulfidptosis and periodontitis: New insights and clinical significance

Authors - Yixin Fan
View Article