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Syntaxin 18 Monoclonal antibody

Syntaxin 18 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A2C9

Cat no : 68220-1-Ig

Synonyms

DKFZp686O15149, STX18, syntaxin 18, Ufe1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Syntaxin 18 Monoclonal antibody (68220-1-Ig)

    WB analysis using 68220-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68220-1-Ig (Syntaxin 18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of various lysates using Syntaxin 18 Monoclonal antibody (68220-1-Ig)

    WB analysis using 68220-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68220-1-Ig (Syntaxin 18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using Syntaxin 18 Monoclonal antibody (68220-1-Ig)

    WB analysis of pig brain using 68220-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 68220-1-Ig (Syntaxin 18 antibody) at dilution of 1:9800 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inLNCaP cells, rat brain tissue, pig brain tissue, HEK-293 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68220-1-Ig targets Syntaxin 18 in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Syntaxin 18 fusion protein Ag8947
Full Name syntaxin 18
Calculated Molecular Weight 335 aa, 39 kDa
Observed Molecular Weight39 kDa
GenBank Accession NumberBC014613
Gene Symbol Syntaxin 18
Gene ID (NCBI) 53407
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syntaxin 18 is a novel member of the syntaxin family and it is involved in ER-mediated phagocytosis by regulating the specific and direct fusion of the ER and plasma or phagosomal membranes( PMID: 16790498).

Protocols

Product Specific Protocols
WB protocol for Syntaxin 18 antibody 68220-1-IgDownload protocol
FC protocol for Syntaxin 18 antibody 68220-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols