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NCAM2 Monoclonal antibody

NCAM2 Monoclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rabbit, Mouse, Rat

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1D6C12

Cat no : 68205-1-Ig

Synonyms

N CAM 2, NCAM 2, NCAM2, NCAM21

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NCAM2 Monoclonal antibody (68205-1-Ig)

    WB analysis using 68205-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68205-1-Ig (NCAM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using NCAM2 Monoclonal antibody (68205-1-Ig)

    WB analysis of LNCaP using 68205-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68205-1-Ig (NCAM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using NCAM2 Monoclonal antibody (68205-1-Ig)

    IF Staining of mouse brain using 68205-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NCAM2 antibody (68205-1-Ig, Clone: 1D6C12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using NCAM2 Monoclonal antibody (68205-1-Ig)

    IF Staining of mouse brain using 68205-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NCAM2 antibody (68205-1-Ig, Clone: 1D6C12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inrabbit brain tissue, LNCaP cells, rat brain tissue, mouse brain tissue, rat cerebellum tissue, mouse cerebellum tissue
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68205-1-Ig targets NCAM2 in WB, IF-P, ELISA applications and shows reactivity with Human, Rabbit, Mouse, Rat samples.

Tested Reactivity Human, Rabbit, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NCAM2 fusion protein Ag4935
Full Name neural cell adhesion molecule 2
Calculated Molecular Weight 93 kDa
Observed Molecular Weight93 kDa, 125 kDa
GenBank Accession NumberBC052946
Gene Symbol NCAM2
Gene ID (NCBI) 4685
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NCAM2 antibody 68205-1-IgDownload protocol
IF protocol for NCAM2 antibody 68205-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols