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CYP2S1 Monoclonal antibody

CYP2S1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3E5D4

Cat no : 68198-1-Ig

Synonyms

CYP2S1, CYPIIS1, Cytochrome P450 2S1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CYP2S1 Monoclonal antibody (68198-1-Ig)

    WB analysis using 68198-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68198-1-Ig (CYP2S1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using CYP2S1 Monoclonal antibody (68198-1-Ig)

    WB analysis using 68198-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68198-1-Ig (CYP2S1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inNCCIT cells, HeLa cells, A549 cells, pig colon tissue, pig rectum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68198-1-Ig targets CYP2S1 in WB, ELISA applications and shows reactivity with Human, Pig samples.

Tested Reactivity Human, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CYP2S1 fusion protein Ag4605
Full Name cytochrome P450, family 2, subfamily S, polypeptide 1
Calculated Molecular Weight 504 aa, 56 kDa
Observed Molecular Weight50-56 kDa
GenBank Accession NumberBC033691
Gene Symbol CYP2S1
Gene ID (NCBI) 29785
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The cytochrome P450 (CYP) family of isoenzymes are involved in the metabolism of diverse endogenous compounds and detoxification of exogenous agents. Cytochrome P450 2S1 (CYP2S1) is one 'orphan' CYP, and is particularly expressed in epithe-lial cells of tissues which are exposed to the environment such as the skin, respiratory, gastrointes-tinal, and urinary tracts. CYP2S1 is associated primarily with the synthesis and metabo-lism of lipids including prostaglandins (PGE2) and retinoids. In addition, CYP2S1 expression is associated with patient survival in breast cancer.

Protocols

Product Specific Protocols
WB protocol for CYP2S1 antibody 68198-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols