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VPS25 Monoclonal antibody

VPS25 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rabbit

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3B4C3

Cat no : 68197-1-Ig

Synonyms

DERP9, EAP20, ESCRT II complex subunit VPS25, FAP20, hVps25, VPS25

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VPS25 Monoclonal antibody (68197-1-Ig)

    WB analysis using 68197-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68197-1-Ig (VPS25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rabbit kidney tissue using VPS25 Monoclonal antibody (68197-1-Ig)

    WB analysis of rabbit kidney using 68197-1-Ig

    rabbit kidney tissue were subjected to SDS PAGE followed by western blot with 68197-1-Ig (VPS25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using VPS25 Monoclonal antibody (68197-1-Ig)

    WB analysis of HeLa using 68197-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68197-1-Ig (VPS25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using VPS25 Monoclonal antibody (68197-1-Ig)

    IF Staining of HepG2 using 68197-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VPS25 antibody (68197-1-Ig, Clone: 3B4C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, rabbit kidney tissue, HepG2 cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68197-1-Ig targets VPS25 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Rabbit samples.

Tested Reactivity Human, Rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VPS25 fusion protein Ag8243
Full Name vacuolar protein sorting 25 homolog (S. cerevisiae)
Calculated Molecular Weight 176 aa, 21 kDa
Observed Molecular Weight19 kDa
GenBank Accession NumberBC006282
Gene Symbol VPS25
Gene ID (NCBI) 84313
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VPS25 is a component of the endosome-associated complex ESCRT-II (Endosomal Sorting Complexes Required for Transport protein II). ESCRT-II complex, composed of VPS25, VPS36, and SNF8, functions in sorting of ubiquitinated membrane proteins during endocytosis. ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. VPS25 is highly conserved. Human VPS25 gene maps to chromosome 17q21.31 and is expressed in a wide range of tissues (PMID: 16889659).

Protocols

Product Specific Protocols
WB protocol for VPS25 antibody 68197-1-IgDownload protocol
IF protocol for VPS25 antibody 68197-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols