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RPAP1 Monoclonal antibody

RPAP1 Monoclonal Antibody for WB, ELISA, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA, FC (Intra)

Conjugate

Unconjugated

CloneNo.

2A6H5

Cat no : 68193-1-Ig

Synonyms

RPAP 1, RNA polymerase II-associated protein 1, KIAA1403, 2A6H5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPAP1 Monoclonal antibody (68193-1-Ig)

    WB analysis using 68193-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68193-1-Ig (RPAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using RPAP1 Monoclonal antibody (68193-1-Ig)

    WB analysis of mouse brain using 68193-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 68193-1-Ig (RPAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using RPAP1 Monoclonal antibody (68193-1-Ig)

    FC experiment of HeLa using 68193-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug RPAP1 Monoclonal antibody (68193-1-Ig, Clone:2A6H5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Tested Applications

Positive WB detected inLNCaP cells, mouse brain tissue, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, rat brain tissue
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68193-1-Ig targets RPAP1 in WB, ELISA, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RPAP1 fusion protein Ag7856
Full Name RNA polymerase II associated protein 1
Calculated Molecular Weight 153 kDa
Observed Molecular Weight 153 kDa
GenBank Accession NumberBC000246
Gene Symbol RPAP1
Gene ID (NCBI) 26015
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNA polymerase II-associated protein 1 (RPAP1) encodes a nuclear 153-kDa protein, and forms an interface between the RNA polymerase II (RNAPII) enzyme and chaperone/scaffolding protein, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation. RPAP1 is required for interaction of the RNA polymerase II complex with acetylated histone H3 and the subsequent DNA transcription.

Protocols

Product Specific Protocols
WB protocol for RPAP1 antibody 68193-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols