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PSMD7 Monoclonal antibody

PSMD7 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3E10E7

Cat no : 68188-1-Ig

Synonyms

MOV34, Mov34 protein homolog, MOV34L, P40, Proteasome subunit p40, PSMD7, Rpn8, S12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMD7 Monoclonal antibody (68188-1-Ig)

    WB analysis using 68188-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68188-1-Ig (PSMD7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of HeLa cells using PSMD7 Monoclonal antibody (68188-1-Ig)

    WB analysis of HeLa using 68188-1-Ig

    WB result of PSMD7 antibody (68188-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMD7 transfected HeLa cells.

Tested Applications

Positive WB detected inHCT 116 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

68188-1-Ig targets PSMD7 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PSMD7 fusion protein Ag9305
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 7
Calculated Molecular Weight 324 aa, 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC012606
Gene Symbol PSMD7
Gene ID (NCBI) 5713
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Proteasome 26S subunit, non-ATPase 7 (PSMD7), an ATP-independent component of the 19S regulatory subunit, is a member of the JAMM/MPN domain-associated metallopeptidase (JAMM) DUB family. PSMD7, as a core component of the 26S proteasome, is critical for the degradation of ubiquitinated proteins in the proteasome. PSMD7 arrests cell cycle in the G2/M phase during HIV infection. The molecular weight of PSMD7 is 37 kDa. (PMID: 34512150, 34234864)

Protocols

Product Specific Protocols
WB protocol for PSMD7 antibody 68188-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

CircSEC24B activates autophagy and induces chemoresistance of colorectal cancer via OTUB1-mediated deubiquitination of SRPX2

Authors - Di Wang
View Article