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ARL8A/ARL8B Monoclonal antibody

ARL8A/ARL8B Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, rabbit

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F4C3

Cat no : 68186-1-Ig

Synonyms

ARL8A, GIE2, ARL10B, ADP-ribosylation factor-like protein 8A, ADP-ribosylation factor-like protein 10B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARL8A/ARL8B Monoclonal antibody (68186-1-Ig)

    WB analysis using 68186-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68186-1-Ig (ARL8A/ARL8B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using ARL8A/ARL8B Monoclonal antibody (68186-1-Ig)

    WB analysis using 68186-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68186-1-Ig (ARL8A/ARL8B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ARL8A/ARL8B Monoclonal antibody (68186-1-Ig)

    WB analysis using 68186-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68186-1-Ig (ARL8A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using ARL8A/ARL8B Monoclonal antibody (68186-1-Ig)

    WB analysis of human placenta using 68186-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68186-1-Ig (ARL8A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ARL8A/ARL8B Monoclonal antibody (68186-1-Ig)

    WB analysis using 68186-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68186-1-Ig (ARL8A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ARL8A/ARL8B Monoclonal antibody (68186-1-Ig)

    IF Staining of A549 using 68186-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ARL8A/ARL8B antibody (68186-1-Ig, Clone: 2F4C3 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of C6 cells using ARL8A/ARL8B Monoclonal antibody (68186-1-Ig)

    IF Staining of C6 using 68186-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using ARL8A/ARL8B antibody (68186-1-Ig, Clone: 2F4C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using ARL8A/ARL8B Monoclonal antibody (68186-1-Ig)

    IF Staining of Neuro-2a using 68186-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using ARL8A/ARL8B antibody (68186-1-Ig, Clone: 2F4C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ARL8A/ARL8B Monoclonal antibody (68186-1-Ig)

    IF Staining of HEK-293 using 68186-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using ARL8A/ARL8B antibody (68186-1-Ig, Clone: 2F4C3 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inrabbit brain tissue, LNCaP cells, human placenta tissue, HeLa cells, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inA549 cells, C6 cells, Neuro-2a cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68186-1-Ig targets ARL8A/ARL8B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Reactivity human, mouse, rat, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ARL8A/ARL8B fusion protein Ag10758
Full Name ADP-ribosylation factor-like 8A
Calculated Molecular Weight 186 aa, 21 kDa
Observed Molecular Weight18 kDa, 21 kDa
GenBank Accession NumberBC015408
Gene Symbol ARL8A
Gene ID (NCBI) 127829
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARL8A (ADP-ribosylation factor-like protein 8A) is also named as ARL10B, GIE2 and belongs to the small GTPase superfamily. ARL8A is localized to lysosomes in mammalian cells (PMID: 16537643). In neurons, ARL8A mediates the anterograde axonal long-range transport of presynaptic lysosome-related vesicles that are required for presynaptic biogenesis and synaptic function. ARL8A is upregulated in many cancers such as endometrial cancer (PMID: 32070877), colorectal cancer (PMID: 30546056) and inflammatory breast cancer (PMID: 27648361), and has been associated with poor survival outcomes. ARL8A/ARL8B can be recognized by this antibody.

Protocols

Product Specific Protocols
WB protocol for ARL8A/ARL8B antibody 68186-1-IgDownload protocol
IF protocol for ARL8A/ARL8B antibody 68186-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols