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NSFL1C Monoclonal antibody

NSFL1C Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F12E12

Cat no : 68182-1-Ig

Synonyms

dJ776F14.1, NSFL1 (p97) cofactor (p47), NSFL1 cofactor p47, NSFL1C, p47, p97 cofactor p47, UBX1, UBXD10, UBXN2C

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NSFL1C Monoclonal antibody (68182-1-Ig)

    WB analysis using 68182-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68182-1-Ig (NSFL1C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using NSFL1C Monoclonal antibody (68182-1-Ig)

    WB analysis using 68182-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68182-1-Ig (NSFL1C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using NSFL1C Monoclonal antibody (68182-1-Ig)

    IHC staining of human lung cancer using 68182-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68182-1-Ig (NSFL1C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using NSFL1C Monoclonal antibody (68182-1-Ig)

    IHC staining of human lung cancer using 68182-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68182-1-Ig (NSFL1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using NSFL1C Monoclonal antibody (68182-1-Ig)

    IF Staining of A431 using 68182-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NSFL1C antibody (68182-1-Ig, Clone: 1F12E12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inrat brain tissue, U2OS cells, mouse brain tissue, A549 cells, A431 cells, LNCaP cells, HeLa cells, Jurkat cells, pig brain tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68182-1-Ig targets NSFL1C in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NSFL1C fusion protein Ag8040
Full Name NSFL1 (p97) cofactor (p47)
Calculated Molecular Weight 41 kDa
Observed Molecular Weight41 kDa
GenBank Accession NumberBC002801
Gene Symbol NSFL1C
Gene ID (NCBI) 55968
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NSFL1C antibody 68182-1-IgDownload protocol
IHC protocol for NSFL1C antibody 68182-1-IgDownload protocol
IF protocol for NSFL1C antibody 68182-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols