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PPA1 Monoclonal antibody

PPA1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D11F4

Cat no : 68178-1-Ig

Synonyms

Inorganic pyrophosphatase, IOPPP, PP, PP1, PPA1, PPase, pyrophosphatase (inorganic) 1, SID6 8061

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPA1 Monoclonal antibody (68178-1-Ig)

    WB analysis using 68178-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68178-1-Ig (PPA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PPA1 Monoclonal antibody (68178-1-Ig)

    IF Staining of HeLa using 68178-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PPA1 antibody (68178-1-Ig, Clone: 1D11F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68178-1-Ig targets PPA1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PPA1 fusion protein Ag7049
Full Name pyrophosphatase (inorganic) 1
Calculated Molecular Weight 33 kDa
Observed Molecular Weight33 kDa
GenBank Accession NumberBC001022
Gene Symbol PPA1
Gene ID (NCBI) 5464
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPA1(Inorganic pyrophosphatase) is also named as IOPPP, PP and PPase. It maintains the thermodynamic favorability of these reactions by catalyzing the hydrolysis of pyrophosphates into organic phosphates, which are then exported across the cell membrane(PMID:16300924).The erythrocyte inorganic pyrophosphatase molecule would appear to be a dimer(PMID:4130389).

Protocols

Product Specific Protocols
WB protocol for PPA1 antibody 68178-1-IgDownload protocol
IF protocol for PPA1 antibody 68178-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols