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EIF4E3 Monoclonal antibody

EIF4E3 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E9C4

Cat no : 68171-1-Ig

Synonyms

eIF 4E type 3, eIF 4E3, eIF4E 3, eIF4E type 3, EIF4E3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF4E3 Monoclonal antibody (68171-1-Ig)

    WB analysis using 68171-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68171-1-Ig (EIF4E3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using EIF4E3 Monoclonal antibody (68171-1-Ig)

    IHC staining of mouse colon using 68171-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 68171-1-Ig (EIF4E3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using EIF4E3 Monoclonal antibody (68171-1-Ig)

    IHC staining of mouse colon using 68171-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 68171-1-Ig (EIF4E3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using EIF4E3 Monoclonal antibody (68171-1-Ig)

    IHC staining of rat colon using 68171-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 68171-1-Ig (EIF4E3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using EIF4E3 Monoclonal antibody (68171-1-Ig)

    IHC staining of rat colon using 68171-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 68171-1-Ig (EIF4E3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, MOLT-4 cells, Jurkat cells, THP-1 cells, K-562 cells
Positive IHC detected inrat colon tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68171-1-Ig targets EIF4E3 in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen EIF4E3 fusion protein Ag11056
Full Name eukaryotic translation initiation factor 4E family member 3
Calculated Molecular Weight 24 kDa
Observed Molecular Weight24-30 kDa
GenBank Accession NumberBC031289
Gene Symbol EIF4E3
Gene ID (NCBI) 317649
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EIF4E3, also known as Eukaryotic translation initiation factor 4E type 3, is a 224 amino acid protein, which recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis. EIF4E3 acts as a tissue-specific tumor suppressor. For instance, eIF4E3 inhibits expression of both mRNA export and translation targets of eIF4E1, consistent with its nuclear and cytoplasmic localization. Importantly, eIF4E3 overexpression represses oncogenic transformation. (PMID: 23587918).

Protocols

Product Specific Protocols
WB protocol for EIF4E3 antibody 68171-1-IgDownload protocol
IHC protocol for EIF4E3 antibody 68171-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols