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GNAI3 Monoclonal antibody

GNAI3 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A9B6

Cat no : 68156-1-Ig

Synonyms

87U6, FLJ26559, G(i) alpha 3, GNAI3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GNAI3 Monoclonal antibody (68156-1-Ig)

    WB analysis using 68156-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68156-1-Ig (GNAI3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GNAI3 Monoclonal antibody (68156-1-Ig)

    IF Staining of HeLa using 68156-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GNAI3 antibody (68156-1-Ig, Clone: 1A9B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68156-1-Ig targets GNAI3 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GNAI3 fusion protein Ag32662
Full Name guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3
Calculated Molecular Weight 40 kDa
Observed Molecular Weight~40 kDa
GenBank Accession NumberBC025285
Gene Symbol GNAI3
Gene ID (NCBI) 2773
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GNAI3 is a member of the Gi group proteins, which belong to the Gα protein family. GNAI3 is involved in several critical biological processes and regulates many cellular activities, including proliferation, differentiation, apoptosis, and migration. For example, GNAI3 can regulate a class of K+ channels in response to hormone and neurotransmitter signals. In addition to its function as a downstream signal pathway switch for receptors on the plasma membrane, GNAI3 has also been shown to localize to the centrosome and affect cytokinesis. These indicate that GNAI3 has important receptor-independent functions. The molecular weight of GNAI3 is 40 kDa. (PMID: 25444921, 34803495)

Protocols

Product Specific Protocols
WB protocol for GNAI3 antibody 68156-1-IgDownload protocol
IF protocol for GNAI3 antibody 68156-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols