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UBE2D1/2/3/4 Monoclonal antibody

UBE2D1/2/3/4 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1C7D12

Cat no : 68138-1-Ig

Synonyms

E2(17)KB3, UBC4/5, UBC5C, UBCH5C, UBE2D3, Ubiquitin carrier protein D3, Ubiquitin protein ligase D3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBE2D1/2/3/4 Monoclonal antibody (68138-1-Ig)

    WB analysis using 68138-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68138-1-Ig (UBE2D3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using UBE2D1/2/3/4 Monoclonal antibody (68138-1-Ig)

    WB analysis using 68138-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68138-1-Ig (UBE2D3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using UBE2D1/2/3/4 Monoclonal antibody (68138-1-Ig)

    IF Staining of A431 using 68138-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using UBE2D1/2/3/4 antibody (68138-1-Ig, Clone: 1C7D12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68138-1-Ig targets UBE2D1/2/3/4 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen UBE2D1/2/3/4 fusion protein Ag2278
Full Name ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast)
Calculated Molecular Weight 17 kDa
Observed Molecular Weight17 kDa
GenBank Accession NumberBC003395
Gene Symbol UBE2D3
Gene ID (NCBI) 7323
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquitination is a post-translational modification pathway or is part of the specific protein-degradation pathway by the 26S proteasome. Ubiquitination of a target protein involves multistep enzymatic reaction catalyzed by a cascade of enzymes including Ub-activating enzymes (E1s), Ub-conjugating enzymes (E2s) and Ub ligases (E3s) (PMID: 23542885). UBE2D family, which has 4 members named as UBE2D1/2/3/4, is an E2 ubiquitin-conjugating enzyme family in the ubiquitin-proteasome system. This antibody can recognize all the four members of UBE2D due to the high homology.

Protocols

Product Specific Protocols
WB protocol for UBE2D1/2/3/4 antibody 68138-1-IgDownload protocol
IF protocol for UBE2D1/2/3/4 antibody 68138-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols