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IL-11 Monoclonal antibody

IL-11 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F3A12

Cat no : 68135-1-Ig

Synonyms

IL11, interleukin 11, IL 11, AGIF, Adipogenesis inhibitory factor

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-11 Monoclonal antibody (68135-1-Ig)

    WB analysis using 68135-1-Ig

    Saos-2 cell supernatant (cells stimulated with 500ng/mL HGF for 4 days) were subjected to SDS PAGE followed by western blot with 68135-1-Ig (IL-11 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inSaos-2 supernatant cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68135-1-Ig targets IL-11 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Affinity
KD=1.80 x 10-9M
KOff=2.20 x 10-6M
KOn=1.22 x 103M
Immunogen IL-11 fusion protein Ag11199
Full Name interleukin 11
Calculated Molecular Weight 199 aa, 21 kDa
Observed Molecular Weight21 kDa
GenBank Accession NumberBC012506
Gene Symbol IL-11
Gene ID (NCBI) 3589
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-11 (IL-11) is a member of the glycoprotein-130 (GP-130) cytokines that utilizes the GP-130 signaling pathway shared by other cytokines of the same family. The most well-known role of IL-11 is in hematopoiesis because it promotes megakaryocytopoiesis, erythropoiesis, and thrombopoiesis. In recognition of these properties, human IL-11 recombinant protein (rhIL-11) is used clinically for treating chemotherapy-induced thrombocytopenia. IL-11 has extensive immunomodulatory effects, both anti- and proinflammatory.

Protocols

Product Specific Protocols
WB protocol for IL-11 antibody 68135-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols