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PLA2G4A Monoclonal antibody

PLA2G4A Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2D10H8

Cat no : 68133-1-Ig

Synonyms

cPLA2, cPLA2 alpha, Cytosolic phospholipase A2, PLA2G4, PLA2G4A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLA2G4A Monoclonal antibody (68133-1-Ig)

    WB analysis using 68133-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PLA2G4A Monoclonal antibody (68133-1-Ig)

    WB analysis of HeLa using 68133-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using PLA2G4A Monoclonal antibody (68133-1-Ig)

    WB analysis of NIH/3T3 using 68133-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using PLA2G4A Monoclonal antibody (68133-1-Ig)

    WB analysis of A549 using 68133-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PLA2G4A Monoclonal antibody (68133-1-Ig)

    WB analysis using 68133-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PLA2G4A Monoclonal antibody (68133-1-Ig)

    IHC staining of human lung cancer using 68133-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68133-1-Ig (PLA2G4A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PLA2G4A Monoclonal antibody (68133-1-Ig)

    IHC staining of human lung cancer using 68133-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68133-1-Ig (PLA2G4A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PLA2G4A Monoclonal antibody (68133-1-Ig)

    IF Staining of HeLa using 68133-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PLA2G4A antibody (68133-1-Ig, Clone: 2D10H8 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, A549 cells, HeLa cells, MJ cells, NIH/3T3 cells, K-562 cells, HSC-T6 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68133-1-Ig targets PLA2G4A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PLA2G4A fusion protein Ag32674
Full Name phospholipase A2, group IVA (cytosolic, calcium-dependent)
Calculated Molecular Weight 749 aa, 85 kDa
Observed Molecular Weight85-100 kDa
GenBank Accession NumberBC114340
Gene Symbol PLA2G4A
Gene ID (NCBI) 5321
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLA2G4A, also named cPLA2 (cytosolic phospholipase A2), is a highly conserved and widely expressed enzyme that can promote lipid mediator production. It is present in the cytosol of resting cells and upon activation by a variety of agonists, undergoes Ca++-directed translocation to perinuclear membranes.PLA2G4A plays a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response. It has been reported that the activity and expression levels of PLA2G4A are upregulated in several neurodegenerative diseases. It is a 749 amino acid, 85 kDa protein that migrates at 100-112 kDa in polyacrylamide gels(PMID: 17459165).

Protocols

Product Specific Protocols
WB protocol for PLA2G4A antibody 68133-1-IgDownload protocol
IHC protocol for PLA2G4A antibody 68133-1-IgDownload protocol
IF protocol for PLA2G4A antibody 68133-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols