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Syntenin-1 Monoclonal antibody

Syntenin-1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B1A9

Cat no : 68096-1-Ig

Synonyms

MDA 9, MDA9, Scaffold protein Pbp1, SDCBP, ST1, SYCL, Syndecan binding protein 1, Syntenin 1, TACIP18

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Syntenin-1 Monoclonal antibody (68096-1-Ig)

    WB analysis using 68096-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68096-1-Ig (SDCBP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U2OS cells using Syntenin-1 Monoclonal antibody (68096-1-Ig)

    WB analysis of U2OS using 68096-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 68096-1-Ig (SDCBP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using Syntenin-1 Monoclonal antibody (68096-1-Ig)

    IHC staining of human malignant melanoma using 68096-1-Ig

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 68096-1-Ig (Syntenin-1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using Syntenin-1 Monoclonal antibody (68096-1-Ig)

    IHC staining of human malignant melanoma using 68096-1-Ig

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 68096-1-Ig (Syntenin-1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, U2OS cells, HEK-293 cell, Jurkat cells
Positive IHC detected inhuman malignant melanoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

68096-1-Ig targets Syntenin-1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Syntenin-1 fusion protein Ag18078
Full Name syndecan binding protein (syntenin)
Calculated Molecular Weight 298 aa, 32 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC113674
Gene Symbol Syntenin-1
Gene ID (NCBI) 6386
RRIDAB_2918833
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syntenin-1, also known as SDCBP (syndecan binding protein) and MDA-9 (melanoma differentiation-associated protein 9), was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. Syntenin-1 is a PDZ-domain-containing molecule that has many interaction partners, and regulates transmembrane-receptor trafficking, cell adhesion, tumor-cell metastasis and neuronal-synapse function. Syntenin-1 is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus.

Protocols

Product Specific Protocols
WB protocol for Syntenin-1 antibody 68096-1-IgDownload protocol
IHC protocol for Syntenin-1 antibody 68096-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal degeneration

Authors - Xiaoman Zhu
View Article