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Tested Applications
| Positive WB detected in | HeLa cells, rabbit kidney tissue, HEK293 cells, LNCaP cells, MCF-7 cells, U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
Product Information
68087-1-Ig targets PYCRL in WB, ELISA applications and shows reactivity with Human, Rabbit samples.
| Tested Reactivity | Human, Rabbit |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PYCRL fusion protein Ag31938 Predict reactive species |
| Full Name | pyrroline-5-carboxylate reductase-like |
| Calculated Molecular Weight | 274 aa, 29 kDa |
| Observed Molecular Weight | 26-29 kDa |
| GenBank Accession Number | BC007993 |
| Gene Symbol | PYCRL |
| Gene ID (NCBI) | 65263 |
| RRID | AB_2918824 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q53H96 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PYCRL antibody 68087-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Discov Deubiquitinase USP9x regulates the proline biosynthesis pathway in non-small cell lung cancer
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