• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

NSF Monoclonal antibody

NSF Monoclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, Rabbit, Rat, Mouse, Chicken

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2B6H6

Cat no : 68061-1-Ig

Synonyms

NEM sensitive fusion protein, NSF, SKD2, Vesicle fusing ATPase, Vesicular fusion protein NSF

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using NSF Monoclonal antibody (68061-1-Ig)

    WB analysis using 68061-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-12 cells using NSF Monoclonal antibody (68061-1-Ig)

    WB analysis of PC-12 using 68061-1-Ig

    PC-12 cells were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using NSF Monoclonal antibody (68061-1-Ig)

    WB analysis of HEK-293 using 68061-1-Ig

    WB result of NSF antibody (68061-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSF transfected HEK-293 cells.

  • Western Blot (WB) analysis of SH-SY5Y cells using NSF Monoclonal antibody (68061-1-Ig)

    WB analysis of SH-SY5Y using 68061-1-Ig

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using NSF Monoclonal antibody (68061-1-Ig)

    IF Staining of mouse brain using 68061-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NSF antibody (68061-1-Ig, Clone: 2B6H6 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inpig brain tissue, SH-SY5Y cells, PC-12 cells, HEK-293 cells, Rabbit brain tissue, Rat brain tissue, Mouse brain tissue, Chicken brain tissue
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68061-1-Ig targets NSF in WB, IF-P, ELISA applications and shows reactivity with Human, Pig, Rabbit, Rat, Mouse, Chicken samples.

Tested Reactivity Human, Pig, Rabbit, Rat, Mouse, Chicken
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NSF fusion protein Ag15422
Full Name N-ethylmaleimide-sensitive factor
Calculated Molecular Weight 744 aa, 83 kDa
Observed Molecular Weight70-83 kDa
GenBank Accession NumberBC030613
Gene Symbol NSF
Gene ID (NCBI) 4905
RRIDAB_2918802
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NSF antibody 68061-1-IgDownload protocol
IF protocol for NSF antibody 68061-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols