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RPL9 Monoclonal antibody

RPL9 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2A6B11

Cat no : 68054-1-Ig

Synonyms

60S ribosomal protein L9, DKFZp313J1510, FLJ27456, ribosomal protein L9, RPL9, RPL9P7, RPL9P8, RPL9P9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPL9 Monoclonal antibody (68054-1-Ig)

    WB analysis using 68054-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68054-1-Ig (RPL9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using RPL9 Monoclonal antibody (68054-1-Ig)

    IHC staining of mouse cerebellum using 68054-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68054-1-Ig (RPL9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using RPL9 Monoclonal antibody (68054-1-Ig)

    IHC staining of mouse cerebellum using 68054-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68054-1-Ig (RPL9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using RPL9 Monoclonal antibody (68054-1-Ig)

    IF Staining of mouse cerebellum using 68054-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using RPL9 antibody (68054-1-Ig, Clone: 2A6B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using RPL9 Monoclonal antibody (68054-1-Ig)

    IF Staining of mouse cerebellum using 68054-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using RPL9 antibody (68054-1-Ig, Clone: 2A6B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, A549 cells, HepG2 cells, MCF-7 cells, U2OS cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

68054-1-Ig targets RPL9 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityrat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RPL9 fusion protein Ag5391
Full Name ribosomal protein L9
Calculated Molecular Weight 22 kDa
Observed Molecular Weight22 kDa
GenBank Accession NumberBC066318
Gene Symbol RPL9
Gene ID (NCBI) 6133
RRIDAB_2918795
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RPL9 antibody 68054-1-IgDownload protocol
IHC protocol for RPL9 antibody 68054-1-IgDownload protocol
IF protocol for RPL9 antibody 68054-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

J Cardiovasc Transl Res

CREB1 Silencing Protects Against Inflammatory Response in Rats with Deep Vein Thrombosis Through Reducing RPL9 Expression and Blocking NF-κB Signaling

Authors - Xiaorong Jian
View Article