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TTLL12 Monoclonal antibody

TTLL12 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1H5C11

Cat no : 68044-1-Ig

Synonyms

dJ526I14.2, FLJ41795, KIAA0153, TTLL12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TTLL12 Monoclonal antibody (68044-1-Ig)

    WB analysis using 68044-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68044-1-Ig (TTLL12 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TTLL12 Monoclonal antibody (68044-1-Ig)

    IHC staining of human lung cancer using 68044-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68044-1-Ig (TTLL12 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using TTLL12 Monoclonal antibody (68044-1-Ig)

    IHC staining of human ovary tumor using 68044-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68044-1-Ig (TTLL12 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inSKOV-3 cells, LNCap cells, PC-3 cells, A431 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman lung cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68044-1-Ig targets TTLL12 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TTLL12 fusion protein Ag13804
Full Name tubulin tyrosine ligase-like family, member 12
Calculated Molecular Weight 644 aa, 74 kDa
Observed Molecular Weight72-74 kDa
GenBank Accession NumberBC001070
Gene Symbol TTLL12
Gene ID (NCBI) 23170
RRIDAB_2918786
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TTLL12 antibody 68044-1-IgDownload protocol
IHC protocol for TTLL12 antibody 68044-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols