CD35 Monoclonal antibody
CD35 Monoclonal Antibody for IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
1B8E6
Cat no : 68033-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human appendicitis tissue |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:550-1:2200 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
68033-1-Ig targets CD35 in IHC, IF-P, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | CD35 fusion protein Ag30268 |
Full Name | complement component (3b/4b) receptor 1 (Knops blood group) |
Calculated Molecular Weight | 224 kDa |
GenBank Accession Number | NM_000573 |
Gene Symbol | CR1 |
Gene ID (NCBI) | 1378 |
RRID | AB_2918775 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, is a single-chain glycoprotein consisting of 30 repeating homologous protein domains known as short consensus repeats (~60 amino acids each) followed by transmembrane and cytoplasmic domains (PMID: 8765013). CD35 is variably expressed by granulocytes, monocytes, B cells, some T cells, erythrocytes, follicular dendritic cells, Langerhans cells, and glomerular podocytes (PMID: 19004497). CD35 acts as a receptor for C3b and C4b and plays important roles in the regulation of the complement cascade and clearance of immune complexes. A soluble form of CD35 has been found in plasma (PMID: 3156931). It has been reported that soluble CD35 is produced by a proteolytic cleavage in the C-terminal region of CD35 transmembrane domain (PMID: 9405292).
Protocols
Product Specific Protocols | |
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IHC protocol for CD35 antibody 68033-1-Ig | Download protocol |
IF protocol for CD35 antibody 68033-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |