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Phospho-MEK1 (Thr386) Monoclonal antibody

Phospho-MEK1 (Thr386) Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G6A2

Cat no : 68015-1-Ig

Synonyms

ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MAPKK1, MEK 1, MEK1, MKK1, Phospho-MEK1 (Thr386), PRKMK1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-MEK1 (Thr386) Monoclonal antibody (68015-1-Ig)

    WB analysis using 68015-1-Ig

    Non-treated NIH/3T3 cells and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68015-1-Ig (Phospho-MEK1 (Thr386) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • Western Blot (WB) analysis of HeLa cells using Phospho-MEK1 (Thr386) Monoclonal antibody (68015-1-Ig)

    WB analysis of HeLa using 68015-1-Ig

    Non-treated HeLa cells and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 68015-1-Ig (Phospho-MEK1 (Thr386) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-MEK1 (Thr386) Monoclonal antibody (68015-1-Ig)

    WB analysis using 68015-1-Ig

    Non-treated HeLa cells, phosphatase inhibitor treated and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 68015-1-Ig (Phospho-MEK1 (Thr386) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Phospho-MEK1 (Thr386) Monoclonal antibody (68015-1-Ig)

    WB analysis using 68015-1-Ig

    Non-treated HEK-293 cells and nocodazole treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 68015-1-Ig (Phospho-MEK1 (Thr386) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-MEK1 (Thr386) Monoclonal antibody (68015-1-Ig)

    WB analysis using 68015-1-Ig

    Non-treated A431 cells and nocodazole treated A431 cells were subjected to SDS PAGE followed by western blot with 68015-1-Ig (Phospho-MEK1 (Thr386) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, A431 cells, NIH/3T3 cells, nocodazole treated HEK-293 cells, nocodazole treated A431 cells, Calyculin A treated NIH/3T3 cells, Calyculin A treated HeLa cells, λ phosphatase treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

68015-1-Ig targets Phospho-MEK1 (Thr386) in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name mitogen-activated protein kinase kinase 1
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 40-50 kDa
GenBank Accession NumberBC139729
Gene Symbol MEK1
Gene ID (NCBI) 5604
RRIDAB_2918761
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAP2K1 encodes MAPK1, also known as MEK1. MEK1 variants can enhance MEK1 expression and ERK1 phosphorylation that together lead to continuous activation of MEK/ERK signaling pathway. MEK1 bind directly to ERK2 through a region in the N terminus of MEK. In addition, a proline-rich (PR) regulatory sequence in MEK is also involved in MEK-ERK association and signal propagation. The coupling between MEK1 and ERK2 is enhanced through phosphorylation on S298 in the MEK1 PR region, whereas phosphorylation on MEK1 T292 releases the complex. MEK1 T292 is a substrate of ERK2, but the site is also phosphorylated at a basal level when ERK2 is inhibited, suggesting several regulators of this site . Although the S298 site in MEK2 has been conserved, it lacks the T292 phosphorylation site, and it is not a substrate of PAK1. (PMID: 31972311, PMID: 17928366, PMID: 22177953)

Protocols

Product Specific Protocols
WB protocol for Phospho-MEK1 (Thr386) antibody 68015-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB

Cell Rep

Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism

Authors - Hao Qin
View Article