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BCAS2 Monoclonal antibody

BCAS2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A12G1

Cat no : 68003-1-Ig

Synonyms

BCAS2, DAM1, Pre mRNA splicing factor SPF27

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BCAS2 Monoclonal antibody (68003-1-Ig)

    WB analysis using 68003-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68003-1-Ig (BCAS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of LNCaP cells using BCAS2 Monoclonal antibody (68003-1-Ig)

    WB analysis of LNCaP using 68003-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68003-1-Ig (BCAS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using BCAS2 Monoclonal antibody (68003-1-Ig)

    WB analysis of HeLa using 68003-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68003-1-Ig (BCAS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using BCAS2 Monoclonal antibody (68003-1-Ig)

    WB analysis of HEK-293 using 68003-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 68003-1-Ig (BCAS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHepG2 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68003-1-Ig targets BCAS2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BCAS2 fusion protein Ag21309
Full Name breast carcinoma amplified sequence 2
Calculated Molecular Weight 26 kDa
Observed Molecular Weight28 kDa
GenBank Accession NumberBC005285
Gene Symbol BCAS2
Gene ID (NCBI) 10286
RRIDAB_2918750
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Breast carcinoma amplified sequence 2 (BCAS2) is preferentially known as pre-mRNA splicing factor SPF27 and was originally characterized as an up-regulated gene by amplification in human breast cancer cells. BCAS2 has been shown to be involved in DNA damage repair through the replication protein A (RPA) complex.

Protocols

Product Specific Protocols
WB protocol for BCAS2 antibody 68003-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols