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Alpha B Crystallin Monoclonal antibody

Alpha B Crystallin Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1B4C3

Cat no : 68001-1-Ig

Synonyms

Alpha crystallin B chain, Alpha(B) crystallin, CRYA2, CRYAB, crystallin, alpha B, CTPP2, Heat shock protein beta 5, HSPB5, Rosenthal fiber component

Validation Data Gallery

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  • Western Blot (WB) analysis of rat eye tissue using Alpha B Crystallin Monoclonal antibody (68001-1-Ig)

    WB analysis of rat eye using 68001-1-Ig

    rat eye tissue were subjected to SDS PAGE followed by western blot with 68001-1-Ig (aB-Crystallin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse eye tissue using Alpha B Crystallin Monoclonal antibody (68001-1-Ig)

    WB analysis of mouse eye using 68001-1-Ig

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 68001-1-Ig (aB-Crystallin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using Alpha B Crystallin Monoclonal antibody (68001-1-Ig)

    IHC staining of mouse eye using 68001-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 68001-1-Ig (Alpha B Crystallin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using Alpha B Crystallin Monoclonal antibody (68001-1-Ig)

    IHC staining of mouse eye using 68001-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 68001-1-Ig (Alpha B Crystallin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse eye tissue, rat eye tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IPSee 1 publications below

Product Information

68001-1-Ig targets Alpha B Crystallin in WB, IHC, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Alpha B Crystallin fusion protein Ag8656
Full Name crystallin, alpha B
Calculated Molecular Weight 175 aa, 20 kDa
Observed Molecular Weight20-22 kDa
GenBank Accession NumberBC007008
Gene Symbol Alpha B Crystallin
Gene ID (NCBI) 1410
RRIDAB_2918749
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha B-crystallin, encoded by CRYAB gene, is multifunctional, serving as both a major structural protein in the lens and a small heat-shock protein in other tissues in mammals. Alpha B-crystallin may contribute to the transparency and refractive index of the lens. Single nucleotide polymorphisms (SNPs) in the promoter region of CRYAB gene have been associated with in multiple sclerosis. Mutations in the CRYAB gene cause distinct clinical phenotypes including isolated posterior polar cataract, myofibrillar myopathy, cardiomyopathy, or a multisystemic disorder combining all these features. Impairment of alpha-B crystallin dimerization may be relevant to the pathogenesis of these disorders.

Protocols

Product Specific Protocols
WB protocol for Alpha B Crystallin antibody 68001-1-IgDownload protocol
IHC protocol for Alpha B Crystallin antibody 68001-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP

Aging (Albany NY)

CRYAB suppresses ferroptosis and promotes osteogenic differentiation of human bone marrow stem cells via binding and stabilizing FTH1

Authors - Bo Tian
View Article