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RPA2 Monoclonal antibody

RPA2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, rabbit

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B9H11

Cat no : 67999-1-Ig

Synonyms

REPA2, Replication factor A protein 2, replication protein A2, 32kDa, RF A protein 2, RP A p32, RP A p34, RPA2, RPA32, RPA34

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPA2 Monoclonal antibody (67999-1-Ig)

    WB analysis using 67999-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of pig spleen tissue using RPA2 Monoclonal antibody (67999-1-Ig)

    WB analysis of pig spleen using 67999-1-Ig

    pig spleen tissue were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat testis tissue using RPA2 Monoclonal antibody (67999-1-Ig)

    WB analysis of rat testis using 67999-1-Ig

    rat testis tissue were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using RPA2 Monoclonal antibody (67999-1-Ig)

    WB analysis of mouse testis using 67999-1-Ig

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rabbit spleen tissue using RPA2 Monoclonal antibody (67999-1-Ig)

    WB analysis of rabbit spleen using 67999-1-Ig

    rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using RPA2 Monoclonal antibody (67999-1-Ig)

    IF Staining of A431 using 67999-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RPA2 antibody (67999-1-Ig, Clone: 1B9H11 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), (CL594-Phalloidin, red).

Tested Applications

Positive WB detected inLNCaP cells, pig spleen tissue, rat testis tissue, mouse testis tissue, rabbit spleen tissue, Hela cells, MOLT-4 cells, Jurkat cells, THP-1 cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67999-1-Ig targets RPA2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, rabbit samples.

Tested Reactivity Human, Mouse, Rat, Pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RPA2 fusion protein Ag24783
Full Name replication protein A2, 32kDa
Calculated Molecular Weight 29 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC001630
Gene Symbol RPA2
Gene ID (NCBI) 6118
RRIDAB_2918747
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPA2, a subunit of the heterotrimeric replication protein A (RPA) complex, is overexpressed in various cancers. RPA2 is a major target for upstream kinases, including CDKs and the three PIKKs (ATR, ataxia-telangiectasia mutated [ATM], and DNA-PK). RPA2 comprises multiple critical Ser/Thr residues that are phosphorylated in sequential order in response to genotoxic stress.

Protocols

Product Specific Protocols
WB protocol for RPA2 antibody 67999-1-IgDownload protocol
IF protocol for RPA2 antibody 67999-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols