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DHPS Monoclonal antibody

DHPS Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E4F5

Cat no : 67998-1-Ig

Synonyms

deoxyhypusine synthase, DHPS, DHS, DS, MIG13

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DHPS Monoclonal antibody (67998-1-Ig)

    WB analysis using 67998-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67998-1-Ig (DHPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using DHPS Monoclonal antibody (67998-1-Ig)

    WB analysis of LNCaP using 67998-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67998-1-Ig (DHPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DHPS Monoclonal antibody (67998-1-Ig)

    WB analysis of HeLa using 67998-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67998-1-Ig (DHPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHEK-293 cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67998-1-Ig targets DHPS in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DHPS fusion protein Ag31046
Full Name deoxyhypusine synthase
Calculated Molecular Weight 41 kDa
GenBank Accession NumberBC014016
Gene Symbol DHPS
Gene ID (NCBI) 1725
RRIDAB_2918746
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DHPS(Deoxyhypusine synthase) catalyzes the conversion of lysine to deoxyhypusine residue on the eukaryotic initiation factor 5A (eIF-5A) precursor using spermidine as the substrate. It belongs to the deoxyhypusine synthase family. This protein has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for DHPS antibody 67998-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols