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Tested Applications
| Positive WB detected in | LNCaP cells, HeLa cells, A549 cells, HEK-293 cells, Jurkat cells, HepG2 cells, K-562 cells, SW480 cells, MCF-7 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67985-1-Ig targets MON1B in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | MON1B fusion protein Ag9920 Predict reactive species |
| Full Name | MON1 homolog B (yeast) |
| Calculated Molecular Weight | 547 aa, 59 kDa |
| Observed Molecular Weight | 59 kDa |
| GenBank Accession Number | BC024277 |
| Gene Symbol | MON1B |
| Gene ID (NCBI) | 22879 |
| RRID | AB_2918734 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q7L1V2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Vacuolar fusion protein MON1 homolog B (MON1B) is also named as HSRG1, KIAA0872 and SAND2, and belongs to the MON1/SAND family. The membrane recruitment of MON1B, a homolog of yeast MON1 protein in mammals, is essential for vesicular fusion of early endosomes (PMID:26987402).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MON1B antibody 67985-1-Ig | Download protocol |
| IF protocol for MON1B antibody 67985-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
