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RNASEH2A Monoclonal antibody

RNASEH2A Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A2E8

Cat no : 67976-1-Ig

Synonyms

AGS4, JUNB, Ribonuclease H2 subunit A, ribonuclease H2, subunit A, Ribonuclease HI large subunit, Ribonuclease HI subunit A, RNase H(35), RNase H2 subunit A, RNase HI large subunit, RNASEH2A, RNASEHI, RNHIA, RNHL

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNASEH2A Monoclonal antibody (67976-1-Ig)

    WB analysis using 67976-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67976-1-Ig (RNASEH2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inLNCaP cells, Hela cells, MCF-7 cells, HEK-293 cells, Jurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67976-1-Ig targets RNASEH2A in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RNASEH2A fusion protein Ag9256
Full Name ribonuclease H2, subunit A
Calculated Molecular Weight 299 aa, 33 kDa
GenBank Accession NumberBC011748
Gene Symbol RNASEH2A
Gene ID (NCBI) 10535
RRIDAB_2918725
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNASEH2A(Ribonuclease H2 subunit A) is also named as RNASEHI, RNHIA and belongs to the RNase HII family and Eukaryotic subfamily. It is involved in the removal of the initiator RNA primers of Okazaki fragments, an essential step during chromosomal DNA replication of the lagging strand(PMID:9482870). It is an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Defects in RNASEH2A are the cause of Aicardi-Goutieres syndrome type 4 (AGS4).

Protocols

Product Specific Protocols
WB protocol for RNASEH2A antibody 67976-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols