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IPO5 Monoclonal antibody

IPO5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E2B9

Cat no : 67962-1-Ig

Synonyms

DKFZp686O1576, FLJ43041, IMB3, Imp5, importin 5, Importin subunit beta 3, IPO5, Karyopherin beta 3, KPNB3, Ran binding protein 5, RANBP5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IPO5 Monoclonal antibody (67962-1-Ig)

    WB analysis using 67962-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67962-1-Ig (IPO5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using IPO5 Monoclonal antibody (67962-1-Ig)

    IHC staining of mouse testis using 67962-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67962-1-Ig (IPO5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using IPO5 Monoclonal antibody (67962-1-Ig)

    IF Staining of A431 using 67962-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using IPO5 antibody (67962-1-Ig, Clone: 1E2B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67962-1-Ig targets IPO5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IPO5 fusion protein Ag30955
Full Name importin 5
Observed Molecular Weight124 kDa
GenBank Accession NumberBC001497
Gene Symbol IPO5
Gene ID (NCBI) 3843
RRIDAB_2918713
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IPO5 is a member of the importin beta family. Structurally, the protein adopts the shape of a right-hand solenoid and is composed of 24 HEAT repeats. IPO5 facilitates cytoplasmic polyadenylation element-binding protein CPEB3 translocation by binding to the RRM1 motif of CPEB3 in neurons. NMDAR signaling increases RanBP1 expression and reduces the level of cytoplasmic GTP-bound Ran. These changes enhance CPEB3-IPO5 interaction, which consequently accelerates the nuclear import of CPEB3 and promotes its nuclear function.

Protocols

Product Specific Protocols
WB protocol for IPO5 antibody 67962-1-IgDownload protocol
IHC protocol for IPO5 antibody 67962-1-IgDownload protocol
IF protocol for IPO5 antibody 67962-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols