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  • Featured Product
  • KD/KO Validated

SATB2 Monoclonal antibody

SATB2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F8E2

Cat no : 67958-1-Ig

Synonyms

KIAA1034, FLJ32076, FLJ21474, DNA-binding protein SATB2, DNA binding protein SATB2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SATB2 Monoclonal antibody (67958-1-Ig)

    WB analysis using 67958-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67958-1-Ig (SATB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using SATB2 Monoclonal antibody (67958-1-Ig)

    WB analysis of NIH/3T3 using 67958-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67958-1-Ig (SATB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SATB2 Monoclonal antibody (67958-1-Ig)

    WB analysis of HEK-293 using 67958-1-Ig

    WB result of SATB2 antibody (67958-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SATB2 transfected HEK-293 cells.

  • Western Blot (WB) analysis of fetal human brain tissue using SATB2 Monoclonal antibody (67958-1-Ig)

    WB analysis of fetal human brain using 67958-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 67958-1-Ig (SATB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SW480 cells using SATB2 Monoclonal antibody (67958-1-Ig)

    WB analysis of SW480 using 67958-1-Ig

    SW480 cells were subjected to SDS PAGE followed by western blot with 67958-1-Ig (SATB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SATB2 Monoclonal antibody (67958-1-Ig)

    WB analysis using 67958-1-Ig

    HepG2 cell lysates and HepG2 nuclear protein were subjected to SDS PAGE followed by western blot with 67958-1-Ig (SATB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using SATB2 Monoclonal antibody (67958-1-Ig)

    IHC staining of human colon using 67958-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67958-1-Ig (SATB2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SATB2 Monoclonal antibody (67958-1-Ig)

    IHC staining of human colon cancer using 67958-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 67958-1-Ig (SATB2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using SATB2 Monoclonal antibody (67958-1-Ig)

    IHC staining of human colon using 67958-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67958-1-Ig (SATB2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, SW480 cells, NIH/3T3 cells, fetal human brain tissue, K-562 cells
Positive IHC detected inhuman colon tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67958-1-Ig targets SATB2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SATB2 fusion protein Ag17237
Full Name SATB homeobox 2
Calculated Molecular Weight 733 aa, 83 kDa
Observed Molecular Weight85-100 kDa
GenBank Accession NumberBC098136
Gene Symbol SATB2
Gene ID (NCBI) 23314
RRIDAB_2918709
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SATB2, also named as KIAA1034, belongs to the CUT homeobox family. SATB2 binds to DNA at nuclear matrix- or scaffold-associated regions. STAB2 recognizes the sugar-phosphate structure of double-stranded DNA. SATB2 is a transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. SATB2 acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. It is required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. It is a repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. SATB2 may play an important role in palate formation. SATB2 acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation. SATB2 has two isoforms produced by alternative splicing with the MW of 70 kDa and 83 kDa. It can be detected as 85-105 kDa by sumo modification (PMID: 14701874, PMID: 35140581).

Protocols

Product Specific Protocols
WB protocol for SATB2 antibody 67958-1-IgDownload protocol
IHC protocol for SATB2 antibody 67958-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Alessandro (Verified Customer) (12-09-2023)

good Ab for cortical neurons

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Cortical organoids