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RON, MST1R Monoclonal antibody

RON, MST1R Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D8D1

Cat no : 67954-1-Ig

Synonyms

CD136, CDw136, MSP receptor, MST1R, p185 Ron, Protein tyrosine kinase 8, PTK8, RON

Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using RON, MST1R Monoclonal antibody (67954-1-Ig)

    WB analysis of BxPC-3 using 67954-1-Ig

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 67954-1-Ig (RON, MST1R antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RON, MST1R Monoclonal antibody (67954-1-Ig)

    WB analysis of HeLa using 67954-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67954-1-Ig (RON, MST1R antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using RON, MST1R Monoclonal antibody (67954-1-Ig)

    WB analysis of PC-3 using 67954-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 67954-1-Ig (RON, MST1R antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using RON, MST1R Monoclonal antibody (67954-1-Ig)

    FC experiment of HeLa using 67954-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug RON, MST1R Monoclonal antibody (67954-1-Ig, Clone:2D8D1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inBxPC-3 cells, HeLa cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67954-1-Ig targets RON, MST1R in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RON, MST1R fusion protein Ag28289
Full Name macrophage stimulating 1 receptor (c-met-related tyrosine kinase)
Calculated Molecular Weight 152 kDa
Observed Molecular Weight150-185 kDa
GenBank Accession NumberBQ936677 NM_002447
Gene Symbol RON
Gene ID (NCBI) 4486
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RON, MST1R antibody 67954-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols