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  • Western Blot (WB) analysis of various lysates using SLC25A36 Monoclonal antibody (67896-1-Ig)

    WB analysis using 67896-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67896-1-Ig (SLC25A36 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SLC25A36 Monoclonal antibody (67896-1-Ig)

    IHC staining of human breast cancer using 67896-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67896-1-Ig (SLC25A36 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SLC25A36 Monoclonal antibody (67896-1-Ig)

    IHC staining of human breast cancer using 67896-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67896-1-Ig (SLC25A36 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using SLC25A36 Monoclonal antibody (67896-1-Ig)

    IF Staining of A431 using 67896-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SLC25A36 antibody (67896-1-Ig, Clone: 1E5E4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

SLC25A36 Monoclonal antibody

SLC25A36 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67896-1-Ig
Clone No.1E5E4
Publications(1)

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Solute carrier family 25 member 36, 1E5E4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67896-1-Ig targets SLC25A36 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SLC25A36 fusion protein Ag23894 Predict reactive species
Full Name solute carrier family 25, member 36
GenBank Accession NumberBC014064
Gene Symbol SLC25A36
Gene ID (NCBI) 55186
RRIDAB_2918652
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96CQ1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SLC25A36 antibody 67896-1-IgDownload protocol
IHC protocol for SLC25A36 antibody 67896-1-IgDownload protocol
IF protocol for SLC25A36 antibody 67896-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Inherit Metab Dis

Hyperinsulinism / hyperammonemia syndrome caused by biallelic SLC25A36 mutation

Authors - Amit Safran
View Article