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GARS Monoclonal antibody

GARS Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2H9D3

Cat no : 67893-1-Ig

Synonyms

AP 4 A synthetase, CMT2D, DSMAV, GARS, Glycine tRNA ligase, glycyl tRNA synthetase, GlyRS, HMN5, SMAD1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GARS Monoclonal antibody (67893-1-Ig)

    WB analysis using 67893-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67893-1-Ig (GARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of HepG2 cells using GARS Monoclonal antibody (67893-1-Ig)

    WB analysis of HepG2 using 67893-1-Ig

    WB result of GARS antibody (67893-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GARS transfected HepG2 cells.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using GARS Monoclonal antibody (67893-1-Ig)

    IHC staining of human skin cancer using 67893-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67893-1-Ig (GARS antibody) at dilution of 1:2000 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using GARS Monoclonal antibody (67893-1-Ig)

    IHC staining of human skin cancer using 67893-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67893-1-Ig (GARS antibody) at dilution of 1:2000 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using GARS Monoclonal antibody (67893-1-Ig)

    IF Staining of A431 using 67893-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GARS antibody (67893-1-Ig, Clone: 2H9D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inLNCaP cells, HepG2 cells, HeLa cells，HEK-293 cells，HepG2 cells，Jurkat cells，K-562 cells，HSC-T6 cells，NIH/3T3 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67893-1-Ig targets GARS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GARS fusion protein Ag8432
Full Name glycyl-tRNA synthetase
Calculated Molecular Weight 739 aa, 83 kDa
Observed Molecular Weight75-80 kDa
GenBank Accession NumberBC007755
Gene Symbol GARS
Gene ID (NCBI) 2617
RRIDAB_2918649
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycyl-tRNA synthetase 1 (GARS, GeneID:2617) is one of the aminoacyl-tRNA synthetases. It is essential for protein synthesis in all cells and tissues (PMID: 17035524) and charges tRNA molecules with glycine by catalyzing the ATP-dependent ligation of glycine to the 3'-end of it's cognate tRNA via the formation of an aminoacyl-adenylate intermediate (Gly-AMP) (PMID: 17544401, PMID: 28675565, PMID: 24898252) . GARS is located in cytoplasm, but also can be secreted from cells and as an extracellular protein which may influence cell signaling. Mutation of GARS causes peripheral neuropathies such as CMT2D, SMA (PMID: 26503042, PMID: 32181591) , or associates with cancer. Two transcript variants encoding different isoforms have been found for this gene. The molecular weight of isoform2 is about 78 kDa.

Protocols

Product Specific Protocols
WB protocol for GARS antibody 67893-1-IgDownload protocol
IHC protocol for GARS antibody 67893-1-IgDownload protocol
IF protocol for GARS antibody 67893-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols